STAT4 antibody

Catalog No. ABIN7217140

The Rabbit Polyclonal anti-STAT4 antibody is suitable to detect STAT4 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IP and IF.

Quick Overview for STAT4 antibody (ABIN7217140)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-STAT4 Antibody

Purpose: Rabbit Anti-Stat4 Polyclonal Antibody

Specificity: Stat4 Polyclonal Antibody detects endogenous levels of Stat4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Stat4 around the non-phosphorylation site of Y693

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: Stat4

Background: STAT4, Signal transducer and activator of transcription 4Signal transducer and activator of transcription 4 encoded by STAT4 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 85kD

Gene ID: 6775

UniProt: Q14765

Pathways: JAK-STAT Signaling