STAT4 antibody (pTyr693)

Catalog No. ABIN7224514

The Rabbit Polyclonal anti-STAT4 antibody (ABIN7224514) specifically detects STAT4 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for STAT4 antibody (pTyr693) (ABIN7224514)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-STAT4 Antibody

Binding Specificity: pTyr693

Purpose: Rabbit Anti-Stat4 (phospho Tyr693) Polyclonal Antibody

Specificity: Phospho-Stat4 (Y693) Polyclonal Antibody detects endogenous levels of Stat4 protein only when phosphorylated at Y693.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Stat4 Phospho-Tyr693

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: Stat4

Background: STAT4, Signal transducer and activator of transcription 4The protein encoded by STAT4 (signal transducer and activator of transcription 4) is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in STAT4 may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 85kD

Gene ID: 6775

UniProt: Q14765

Pathways: JAK-STAT Signaling