SP100 antibody (AA 250-330)

Catalog No. ABIN7224440

The Rabbit Polyclonal anti-SP100 antibody is suitable to detect SP100 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for SP100 antibody (AA 250-330) (ABIN7224440)

Target: SP100 (SP100 Nuclear Antigen (SP100))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SP100 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SP100 Antibody

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-SP-100 Polyclonal Antibody

Specificity: SP-100 Polyclonal Antibody detects endogenous levels of SP-100 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human SP-100 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SP100

Target: SP100 (SP100 Nuclear Antigen (SP100))

Alternative Name: SP-100

Background: SP100, Nuclear autoantigen Sp-100, Lysp100b, Nuclear dot-associated Sp100 protein, Speckled 100 kDaSP100 (SP100 nuclear antigen) encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for SP100, one of which encodes a high-mobility group protein.

Molecular Weight: 100kD

Gene ID: 6672

UniProt: P23497

Pathways: Retinoic Acid Receptor Signaling Pathway