TEK antibody (pTyr992)

Catalog No. ABIN7218814

The Rabbit Polyclonal anti-TEK antibody has been validated for WB and ELISA. It is suitable to detect TEK in samples from Human and Mouse.

Quick Overview for TEK antibody (pTyr992) (ABIN7218814)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TEK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TEK Antibody

Binding Specificity: pTyr992

Purpose: Tie-2 (phospho Tyr992) Polyclonal Antibody

Specificity: Phospho-Tie-2 (Y992) Polyclonal Antibody detects endogenous levels of Tie-2 protein only when phosphorylated at Y992.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Tie-2 Phospho-Tyr992

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: Tie-2

Background: Rabbit Anti-Tie-2 (phospho Tyr992) Polyclonal Antibody,TEK, TIE2, VMCM, VMCM1, Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor,TEK encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The TEK receptor tyrosine kinase possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.,Angiopoietin-1 receptor

Molecular Weight: observerd band 125kDa

Gene ID: 7010

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding