NFE4 antibody (acLys43)

Catalog No. ABIN7212636

This Rabbit Polyclonal antibody specifically detects NFE4 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for NFE4 antibody (acLys43) (ABIN7212636)

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NFE4 Antibody

Binding Specificity: acLys43

Purpose: Rabbit Anti-NF-E4 (Acetyl Lys43) Polyclonal Antibody

Specificity: Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the human NF-E4 around the acetylation site of K43

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NFE4

Target: NFE4 (Transcription Factor NF-E4 (NFE4))

Alternative Name: NF-E4

Background: NFE4, Transcription factor NF-E4The erythroid-specific protein encoded by NFE4, and the ubiquitous transcription factor CP2, form the stage selector protein(SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG(CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform(22 kDa) acts as an activator, the short isoform(14 kDa) has been shown to repress gamma-globin gene expression. NFE4 is located in an intron of the FBXL13 gene on the opposite strand.

Molecular Weight: 19kD

Gene ID: 58160

UniProt: Q86UQ8