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Vimentin antibody (pSer83)

The Rabbit Polyclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.
Catalog No. ABIN7224924
$338.03
Plus shipping costs $50.00
Shipping to: United States
Delivery in 12 to 16 Business Days

Quick Overview for Vimentin antibody (pSer83) (ABIN7224924)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human, Rat, Mouse

Host

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Rabbit

Clonality

  • 249
  • 195
Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • Binding Specificity

    • 39
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    pSer83

    Purpose

    Rabbit Anti-Vimentin (phospho Ser83) Polyclonal Antibody

    Specificity

    Phospho-Vimentin (S83) Polyclonal Antibody detects endogenous levels of Vimentin protein only when phosphorylated at S83.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    Immunogen

    Synthesized peptide derived from human Vimentin Phospho-Ser83

    Isotype

    IgG
  • Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Expiry Date

    12 months
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    VIM, VimentinVIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.

    Molecular Weight

    54kD

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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