WASF1 antibody (pTyr125)

Catalog No. ABIN7225724

The Rabbit Polyclonal anti-WASF1 antibody is suitable to detect WASF1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for WASF1 antibody (pTyr125) (ABIN7225724)

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-WASF1 Antibody

Binding Specificity: pTyr125

Purpose: Rabbit Anti-WAVE1 (phospho Tyr125) Polyclonal Antibody

Specificity: Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human WAVE1 Phospho-Tyr125

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for WASF1

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Alternative Name: WAVE1

Background: WASF1, KIAA0269, SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1The protein encoded by WASF1 (WAS protein family member 1), a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for WASF1.

Molecular Weight: 70kD

Gene ID: 8936

UniProt: Q92558

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization