ZYX antibody (pSer142)

Catalog No. ABIN7225018

The Rabbit Polyclonal anti-ZYX antibody has been validated for WB and ELISA. It is suitable to detect ZYX in samples from Human.

Quick Overview for ZYX antibody (pSer142) (ABIN7225018)

Target: ZYX (Zyxin (ZYX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZYX Antibody

Binding Specificity: pSer142

Purpose: Zyxin (phospho Ser142) Polyclonal Antibody

Specificity: Phospho-Zyxin (S142) Polyclonal Antibody detects endogenous levels of Zyxin protein only when phosphorylated at S142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Zyxin Phospho-Ser142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: Zyxin

Background: Rabbit Anti-Zyxin (phospho Ser142) Polyclonal Antibody,ZYX, Zyxin, Zyxin-2,Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.,Zyxin

Molecular Weight: observerd band 61kDa

Gene ID: 7791

UniProt: Q15942