HYOU1 antibody

Catalog No. ABIN7232796

The Mouse Monoclonal anti-HYOU1 antibody (Clone 6E3-2C3) (ABIN7232796) specifically detects HYOU1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for HYOU1 antibody (ABIN7232796)

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6E3-2C3

Product Details anti-HYOU1 Antibody

Purpose: Anti-Grp170 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Grp170 (170 kDa).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography

Immunogen: Synthetic peptide sequence derived from human Grp170 (accession no. NP_006389.3)

Isotype: IgG2b

Application Details

Application Notes: ELISA: confirmed with antibody (1-10 μg/mL) binding to Grp170 at 5 μg/mL on the solid phase
Immunoblotting: with antibody at 1.0-2.0 μg/mL. A band of 170 kDa is detected.
These are recommended concentrations
End user should determine optimal concentrations for their applications.
Positive control: HEK293 cell lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1

Background: Hypoxia up-regulated protein 1,Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {PubMed:10037731}.,Grp170 is a major chaperone/stress protein resident in the ER. It is distantly related in sequence to both hsp110 and hsp70 families, but represents the Grp170 stress protein family. Studies have suggested that Grp170 is an important element of the protein processing machinery of the ER. Unlike Hsp110 and Hsp70, Grp170 has two distinct chaperoning domains, a classic beta-sheet peptide binding domain, with some COOH- terminal sequence, and a COOH-terminal alpha-helical domain. Like other members of the Hsp70 superfamily, Grp170 can elicit anti-tumor immune responses most likely by chaperoning antigenic peptide and binding to receptor(s) on antigen-presenting cells (APC).,Endoplasmic reticulum lumen.,150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170

NCBI Accession: NP_006389

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome