DLG4 antibody (Intracellular)

Catalog No. ABIN7887037

The Guinea Pig Polyclonal anti-DLG4 antibody is suitable to detect DLG4 in samples from Human. It has been validated for WB.

Quick Overview for DLG4 antibody (Intracellular) (ABIN7887037)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DLG4 Antibody

Binding Specificity: AA 505-518, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to PSD-95

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Intracellular

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235231)

Blocking Peptide: (ABIN7235231)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: DLG4

Background:

Synonyms: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62

Description: The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein-protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1.PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3.PSD-95 is found at the postsynaptic membrane of excitatory synapses4.PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.

Gene ID: 1742

NCBI Accession: NM_001365

UniProt: P78352

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity