BRAF antibody (pThr401)

Catalog No. ABIN7265819

The Rabbit Monoclonal anti-BRAF antibody has been validated for WB. It is suitable to detect BRAF in samples from Human.

Quick Overview for BRAF antibody (pThr401) (ABIN7265819)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRAF Antibody

Binding Specificity: pThr401

Purpose: Phospho-B-Raf-T401 Rabbit mAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Phosphorylated Antibodies

Purification: Affinity purification

Immunogen: A phospho specific peptide corresponding to residues surrounding T401 of human B Raf

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008],B-RAF1, B-raf, BRAF1, NS7, RAFB1,Apoptosis,Apoptosis_Inhibition of Apoptosis,Cancer,Cell Biology & Developmental Biology,Cell Cycle,Cell Cycle_Cell differentiation,ErbB-HER Signaling Pathway,ESC Pluripotency and Differentiation,G protein signaling,G protein signaling_G-Protein-Coupled Receptors Signaling to MAPK/Erk,IL-6 Receptor Signaling Pathway,Immunology & Inflammation,Kinase,Kinase_Serine/threonine kinases,Kinase_Tyrosine kinases,MAPK-Erk Signaling Pathway,Neuroscience,Protein phosphorylation,Signal Transduction,T Cell Receptor Signaling Pathway,BRAF

Molecular Weight: 85kDa

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy