Topoisomerase II alpha antibody (AA 1050-1150)

Catalog No. ABIN7270853

The Rabbit Polyclonal anti-Topoisomerase II alpha antibody is suitable to detect Topoisomerase II alpha in samples from Human. It has been validated for WB.

Quick Overview for Topoisomerase II alpha antibody (AA 1050-1150) (ABIN7270853)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1050-1150

Purpose: DNA topoisomerase II alpha (TOP2A) Rabbit pAb

Sequence: NQARFILEKI DGKIIIENKP KKELIKVLIQ RGYDSDPVKA WKEAQQKVPD EEENEESDNE KETEKSDSVT DSGPTFNYLL DMPLWYLTKE KKDELCRLRN E

Cross-Reactivity: Human

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) (NP_001058.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.,TOP2A,TOP2,TP2A,Epigenetics & Nuclear Signaling,Cancer,Drug resistance,Topoisomerases,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Centrosome,G2/M DNA Damage Checkpoint,TOP2A

Molecular Weight: 174kDa/177kDa/178kDa/182kDa

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases