Recombinant RFP antibody (Biotin)
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- Target See all RFP Antibodies
- RFP (Red Fluorescent Protein (RFP))
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Discosoma
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- This RFP antibody is conjugated to Biotin
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Specificity
- MRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange
- Purification
- Produced in: E.coli
- Immunogen
- Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.
- Clone
- 2B12
- Top Product
- Discover our top product RFP Primary Antibody
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- Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- RFP (Red Fluorescent Protein (RFP))
- Alternative Name
- mRFP (RFP Products)
- Background
- One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.
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