BFP Fluorescent Protein antibody
Reactivity: Aequorea victoria
WB, IEM, IF, IHC (fro), IHC (p)
Host: Goat
Polyclonal
unconjugated
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- Target
- BFP Fluorescent Protein
- Reactivity
- Aequorea victoria
- Host
- Goat
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity (Details)
- Reacts against Green Fluorescent Protein
- Purification
- Epitope affinity purified
- Immunogen
- Purified recombinant fluorescent protein produced in E. coli.
- Isotype
- IgG
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- Application Notes
- WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
- Comment
-
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
- Restrictions
- For Research Use only
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- Concentration
- 3 mg/mL
- Buffer
- PBS, 20 % glycerol and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
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- Target
- BFP Fluorescent Protein
- Background
- BFP (Blue fluorescent protein) is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. BFP is a ~27 kDa protein that is optimally excited at a 381 nm and has a maximum of emission at 445 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
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