JNK antibody

Catalog No. ABIN7384333

The Rabbit Monoclonal anti-JNK antibody (Clone R09-1G2) (ABIN7384333) specifically detects JNK in WB. The antibody is reactive with Human, Mouse, Rat and Hamster samples.

Quick Overview for JNK antibody (ABIN7384333)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB)

Clone: R09-1G2

Product Details anti-JNK Antibody

Purification: Affinity Purified

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Concentration: 300 μg/mL

Buffer: 50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK

Background: AI849689,c Jun N terminal kinase 1,C-JUN kinase 1,c-Jun N-terminal kinase 1,EC 2.7.11.24,JNK 1,JNK,JNK-46,JNK1A2,JNK21B1/2,MAP kinase 8,MAPK 8,mapk8,Mitogen activated protein kinase 8,Mitogen-activated protein kinase 8,MK08,p54 gamma,Prkm8,Protein kinase JNK1,Protein kinase,mitogen-activated,8,SAPK 1,SAPK gamma,SAPK1,Stress-activated protein kinase 1,Stress-activated protein kinase JNK1,MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1,PRKM8,SAPK1,SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73,mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.

Molecular Weight:

Observed_MW: 46/54kDa

Calculated_MW: 48/53 kDa

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins