UBTF antibody (AA 546-596)

Catalog No. ABIN7452232

This Rabbit Polyclonal antibody specifically detects UBTF in IP. It exhibits reactivity toward Human.

Quick Overview for UBTF antibody (AA 546-596) (ABIN7452232)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-UBTF Antibody

Binding Specificity: AA 546-596

Purpose: Rabbit anti-UBF Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,X. laevis

Purification: Affinity Purified

Immunogen: between AA 546 and 596

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452232 has not performed satisfactorily when used for WB of UBF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of UBF.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBF

Background: Background: Upstream binding factor (UBF) was originally identified as a multi-HMG (high mobility group)-box DNA binding domain protein that could enhance the in vitro assembly of the RNA polymerase I initiation complex. UBF is a transcription factor that recognizes the ribosomal RNA gene promoter required for the expression of 18S, 5.8S, and 28S ribosomal RNAs.

Gene ID: 7343

NCBI Accession: NP_055048

UniProt: P17480