CHEK1 antibody (AA 250-300)

Catalog No. ABIN7452793

This Goat Polyclonal antibody specifically detects CHEK1 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for CHEK1 antibody (AA 250-300) (ABIN7452793)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 250-300

Purpose: Goat anti-Chk1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IP: 5 - 20 μg/mg lysate

WB: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Background: Background: Chk1 functions as a checkpoint protein kinase that is critical to the regulation of the Cdc25a and Cdc25c phosphatases that promote S and G2/M phase progression, respectively. Chk1 kinase activity inhibits cell cycle progression in the presence of DNA damage by phosphorylating Cdc25a and Cdc25c and causing the degradation of Cdc25a and cytoplasmic sequestration of Cdc25c.

Gene ID: 1111

NCBI Accession: NP_001265

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair