PLK1 antibody (AA 325-375)

Catalog No. ABIN7453390

This Rabbit Polyclonal antibody specifically detects PLK1 in WB, IP and IF/ICC. It exhibits reactivity toward Human.

Quick Overview for PLK1 antibody (AA 325-375) (ABIN7453390)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-PLK1 Antibody

Binding Specificity: AA 325-375

Purpose: Rabbit anti-PLK1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 325 and 375

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 2 - 4 μg/mg lysate

WB: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Background: Polo-like kinase 1 (PLK1) activity is central to the progression through mitosis. PLK1 plays an essential role in spindle formation and chromosome segregation. Recent studies suggest that PLK1 is specifically involved in anaphase spindle elongation, Rho localization to the contractile ring and cytokinesis.

Gene ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Cell Division Cycle, M Phase