TP53BP1 antibody (pSer25)

Catalog No. ABIN7453403

The Rabbit Polyclonal anti-TP53BP1 antibody has been validated for WB, IP and IHC (fp). It is suitable to detect TP53BP1 in samples from Human and Mouse.

Quick Overview for TP53BP1 antibody (pSer25) (ABIN7453403)

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TP53BP1 Antibody

Binding Specificity: pSer25

Purpose: Rabbit anti-Phospho 53BP1 (S25) Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: surrounding serine 25

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 1 - 4 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: 53BP1

Background: Background: 53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Gene ID: 7158

NCBI Accession: NP_005648

UniProt: Q12888

Pathways: DNA Damage Repair