NPM1 antibody (AA 250-294)

Catalog No. ABIN7453525

This Rabbit Polyclonal antibody specifically detects NPM1 in WB, IHC (fp) and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for NPM1 antibody (AA 250-294) (ABIN7453525)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Product Details anti-NPM1 Antibody

Binding Specificity: AA 250-294

Purpose: Rabbit anti-NPM1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 250 and 294

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-NPM1 antibody ABIN7452897.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1

Background: Background: Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) P06748].

Gene ID: 4869

NCBI Accession: NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly