JMY antibody (AA 1-50)

Catalog No. ABIN7454459

Product Details anti-JMY Antibody

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Binding Specificity: AA 1-50

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMY antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Purpose: Rabbit anti-JMY Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. Use rabbit anti-JMY antibody A302-573A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for JMY

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Alternative Name: JMY (JMY Products)

Synonyms: WHDC1L3 antibody, junction mediating and regulatory protein, p53 cofactor antibody, junction-mediating and regulatory protein antibody, JMY antibody, jmy antibody, Jmy antibody

Background: Background: Junction-mediating and -regulatory protein (JMY) acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In the nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300 and increases p53/TP53-dependent transcription and apoptosis suggesting an important role in p53/TP53 stress response such as DNA damage. In the cytoplasm, JMY acts as a nucleation-promoting factor for both branched and un-branched actin filaments. It activates the Arp2/3 complex to induce branched actin filament networks and also catalyzes actin polymerization in the absence of Arp2/3, creating un-branched filaments [taken from the Universal Protein Resource (UniProt) Q8N9B5].

Gene ID: 133746

NCBI Accession: NP_689618

UniProt: Q8N9B5

Pathways: Regulation of Actin Filament Polymerization