MCM4 antibody (AA 750-800)

Catalog No. ABIN7454513

The Rabbit Polyclonal anti-MCM4 antibody (ABIN7454513) specifically detects MCM4 in WB and IHC (fp). The antibody is reactive with Human and Mouse samples.

Quick Overview for MCM4 antibody (AA 750-800) (ABIN7454513)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-MCM4 Antibody

Binding Specificity: AA 750-800

Purpose: Rabbit anti-MCM4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 750 and 800

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: Not recommended. Use rabbit anti-MCM4 antibody A300-191A or A300-195A.

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: Background: The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding.

Gene ID: 4173

NCBI Accession: NP_005905

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA