Recombinant SPI1 antibody (AA 220-270)

Catalog No. ABIN7455166

The Rabbit Monoclonal anti-SPI1 antibody is suitable to detect SPI1 in samples from Human and Mouse. It has been validated for WB, IHC, FACS, IP and ICC.

Quick Overview for Recombinant SPI1 antibody (AA 220-270) (ABIN7455166)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR179J

Product Details anti-SPI1 Antibody

Binding Specificity: AA 220-270

Purpose: Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR179J]

Purification: Purified

Immunogen: between AA 220 and 270 (C-term)

Isotype: IgG

Application Details

Application Notes:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 6 μL/mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: PU.1

Background: PU.1 is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. PU.1 binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. [taken from NCBI Entrez Gene (Gene ID: 6688)].

Gene ID: 6688

UniProt: P17947

Pathways: Stem Cell Maintenance