Phospholipase C gamma 1 antibody (pTyr783)

Catalog No. ABIN7464329

The Rabbit Polyclonal anti-Phospholipase C gamma 1 antibody (ABIN7464329) specifically detects Phospholipase C gamma 1 in WB. The antibody is reactive with Human samples.

Quick Overview for Phospholipase C gamma 1 antibody (pTyr783) (ABIN7464329)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: pTyr783

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Tyr783 of human PLCG1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 (Starvation for 16 hr and then 100 ng/ml EGF treatment for 5 min) , NIH-3T3 (starvation for 16 hr and 200 ng/ml EGF treatment for 30 min)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.27 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: phospholipase C gamma 1

Background: Phospholipase C gamma 1 , NCKAP3 , PLC-II , PLC1 , PLC148 , PLCgamma1,The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 149 kDa

Gene ID: 5335

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling