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Vimentin antibody

This Mouse Monoclonal antibody specifically detects Vimentin in WB, ELISA, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.
Catalog No. ABIN7464381
$740.00
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days

Quick Overview for Vimentin antibody (ABIN7464381)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human

Host

  • 217
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Mouse

Clonality

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Monoclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Grade

KO Validated

Clone

GT7812
  • Cross-Reactivity

    Cat, Dog, Human, Mouse, Rat

    Purification

    Affinity purified by Protein G.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

    Isotype

    IgG2a
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: C8D30 , NIH-3T3 , C2C12 , rat brain , 293T , NT2D1

    Validation: Comparison, KO/KD, Orthogonal, Overexpression

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.64 mg/mL

    Buffer

    PBS, No Preservative

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    vimentin

    Background

    Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

    Molecular Weight

    54 kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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