H2AFX antibody (pSer139)

Catalog No. ABIN7464433

The Rabbit Polyclonal anti-H2AFX antibody has been validated for WB, IF, IHC (p), ICC, IHC (fro) and IHC (wm). It is suitable to detect H2AFX in samples from Human.

Quick Overview for H2AFX antibody (pSer139) (ABIN7464433)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116 (UVB treatment 50mJ for 2hr) , HCT116 (UVB treatment 50mJ for 6hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , HCT-116 (30 uM cisplatin treatment for 24 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.16 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2A histone family member X

Background: H2A histone family member X , H2A.X , H2A/X , H2AX,Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Molecular Weight: 15 kDa

Gene ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus