Glutaminase antibody (C-Term)

Catalog No. ABIN7464725

This anti-Glutaminase antibody is a Rabbit Polyclonal antibody detecting Glutaminase in WB, IF, IP, ICC and IHC (p). Suitable for Human.

Quick Overview for Glutaminase antibody (C-Term) (ABIN7464725)

Target: Glutaminase (GLS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-Glutaminase Antibody

Binding Specificity: C-Term

Specificity: Specific for GAC but not KGA isoform

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-MB-231

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: glutaminase

Background: Glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA,Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Molecular Weight: 65 kDa

Gene ID: 2744

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect