SARM1 antibody (C-Term)

Catalog No. ABIN7466589

This Rabbit Polyclonal antibody specifically detects SARM1 in WB, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for SARM1 antibody (C-Term) (ABIN7466589)

Target: SARM1 (Sterile alpha and TIR Motif Containing 1 (SARM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-SARM1 Antibody

Binding Specificity: C-Term

Specificity: Knockdown/Knockout validation was supported by references (PMID:36055989)

Cross-Reactivity: Drosophila melanogaster, Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SARM1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SARM1

Target: SARM1 (Sterile alpha and TIR Motif Containing 1 (SARM1))

Alternative Name: sterile alpha and TIR motif containing 1

Background: Sterile alpha and TIR motif containing 1 , MyD88-5 , SAMD2 , SARM,Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.

Molecular Weight: 79 kDa

Gene ID: 23098

UniProt: Q6SZW1

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades