Glutaminase antibody (C-Term)

Catalog No. ABIN7466665

The Mouse Monoclonal anti-Glutaminase antibody has been validated for WB, IF and ICC. It is suitable to detect Glutaminase in samples from Human.

Quick Overview for Glutaminase antibody (C-Term) (ABIN7466665)

Target: Glutaminase (GLS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glutaminase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT1075

Product Details anti-Glutaminase Antibody

Binding Specificity: C-Term

Specificity: Specific for GAC but not KGA isoform

Cross-Reactivity: Human

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7 , MDA-MB-231

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Glutaminase

Target: Glutaminase (GLS)

Alternative Name: glutaminase

Background: Glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA,Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Molecular Weight: 65 kDa

Gene ID: 2744

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect