DCLK1 antibody

Catalog No. ABIN7467612

This Rabbit Polyclonal antibody specifically detects DCLK1 in WB. It exhibits reactivity toward Human.

Quick Overview for DCLK1 antibody (ABIN7467612)

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCLK1 Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DCLK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse hippocampus , mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.77 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DCLK1

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: doublecortin like kinase 1

Background: Doublecortin like kinase 1 , CL1 , CLICK1 , DCAMKL1 , DCDC3A , DCLK,This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.[provided by RefSeq, Sep 2010]

Molecular Weight: 82 kDa

Gene ID: 9201

UniProt: O15075