ATR antibody (pThr1989)

Catalog No. ABIN7467984

This Mouse Monoclonal antibody specifically detects ATR in WB, IP, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for ATR antibody (pThr1989) (ABIN7467984)

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATR antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: GT222

Product Details anti-ATR Antibody

Binding Specificity: pThr1989

Cross-Reactivity: Human

Purification: Affinity purified by Protein A.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr1989 of human ATR. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116 , HCT116(100 J/m2 UVC and recover for 6hr) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATR

Target: ATR (Ataxia Telangiectasia and Rad3 Related (ATR))

Alternative Name: ATR serine/threonine kinase

Background:

Synonyms: ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

Background: The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq]

Molecular Weight: 301 kDa

Gene ID: 545

UniProt: Q13535

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus