ATM antibody (AA 2577-3056)

Catalog No. ABIN7467993

This Mouse Monoclonal antibody specifically detects ATM in WB, IHC, ELISA, IF, IP, IHC (p), FACS, ICC and ChIP. It exhibits reactivity toward Human.

Quick Overview for ATM antibody (AA 2577-3056) (ABIN7467993)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Grade: KO Validated

Clone: 2C1

Product Details anti-ATM Antibody

Binding Specificity: AA 2577-3056

Cross-Reactivity: Human, Monkey, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Isotype: IgG1

Application Details

Application Notes: Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/mL.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Comment:

Positive Control: SK-N-SH , HeLa , HeLa nuclear extract Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM serine/threonine kinase

Background:

Synonyms: ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

Background: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates, thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Molecular Weight: 351 kDa

Gene ID: 472

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus