VAV1 antibody

Catalog No. ABIN7469172

This Mouse Monoclonal antibody specifically detects VAV1 in WB, IHC (p), IF and ICC. It exhibits reactivity toward Human.

Quick Overview for VAV1 antibody (ABIN7469172)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VAV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: GT557

Product Details anti-VAV1 Antibody

Cross-Reactivity: Human, Mouse

Purification: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the center region of human VAV1. The exact sequence is proprietary.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat , NCI-H929

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Alternative Name: vav guanine nucleotide exchange factor 1

Background:

Synonyms: vav guanine nucleotide exchange factor 1 , VAV

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Molecular Weight: 98 kDa

Gene ID: 7409

UniProt: P15498

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling