RENT2/UPF2 antibody (C-Term)

Catalog No. ABIN7469647

Product Details anti-RENT2/UPF2 Antibody

Target: RENT2/UPF2 (UPF2) (UPF2 Regulator of Nonsense Transcripts 2 (UPF2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RENT2/UPF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Rent2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RENT2/UPF2

Target: RENT2/UPF2 (UPF2) (UPF2 Regulator of Nonsense Transcripts 2 (UPF2))

Alternative Name: Rent2 (UPF2 Products)

Synonyms: HUPF2 antibody, RENT2 antibody, smg-3 antibody, UPF2, regulator of nonsense mediated mRNA decay antibody, UPF2 regulator of nonsense transcripts homolog (yeast) antibody, UPF2 antibody, Upf2 antibody, upf2 antibody

Background:

Synonyms: UPF2 regulator of nonsense mediated mRNA decay , HUPF2 , RENT2 , smg-3

Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene, both variants encode the same protein. [provided by RefSeq]

Molecular Weight: 148 kDa

Gene ID: 26019

UniProt: Q9HAU5