Recombinant Androgen Receptor antibody (AA 1-100)

Catalog No. ABIN7477375

This anti-Androgen Receptor antibody is a Rabbit Monoclonal antibody detecting Androgen Receptor in IHC. Suitable for Human.

Quick Overview for Recombinant Androgen Receptor antibody (AA 1-100) (ABIN7477375)

Target: Androgen Receptor (AR)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-367R

Product Details anti-Androgen Receptor Antibody

Binding Specificity: AA 1-100

Immunogen: Synthetic peptide corresponding to residues (within aa1-100) of Androgen Receptor.

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: An at least weak to moderate nuclear AR immunostaining should be seen in a subset of kidney tubuli and of glomerular cells.

Negative Control: Colon: AR immunostaining should be absent in colonic mucosa.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background: AIS, Dihydrotestosterone receptor (DHTR), HUMARA, HYSP1, Kennedy disease (KD), Nuclear receptor subfamily 3 group C member 4 (NR3C4), SMAX1, Spinal and bulbar muscular atrophy (SBMA), Testicular Feminization (TFM),Androgen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding