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Recombinant CD22 antibody (AA 52-178)

The Rabbit Monoclonal anti-CD22 antibody is suitable to detect CD22 in samples from Human. It has been validated for IHC.

IHC

CD22 positive B lymphocytes predominate in the white pulp but are only sparse in the red pulp (Recombinant CD22 antibody (AA 52-178))

IHC

CD22 positive B lymphocytes are rare in this sample of ileum mucosa (Recombinant CD22 antibody (AA 52-178))

IHC

3 images

Catalog No. ABIN7470582

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Quick Overview for Recombinant CD22 antibody (AA 52-178) (ABIN7470582)

Target

See all CD22 Antibodies

CD22 (CD22 Molecule (CD22))

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This CD22 antibody is un-conjugated

Application

Immunohistochemistry (IHC)

Clone

022R

Discover our top product CD22 Primary Antibody

Binding Specificity
30

26

11

8

6

4

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 52-178

Purpose

CD22 antibody (022R)

Immunogen

Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Isotype

IgG
anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 Reactivity: Human FACS, WB, ELISA, IHC, ICC Host: Mouse Monoclonal 2H1C4 unconjugated

anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 Reactivity: Human FACS, ELISA, ICC Host: Mouse Monoclonal 1A3A11 unconjugated

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat, StM Host: Mouse Monoclonal BLCAM-1795 unconjugated

anti-CD22 Molecule (CD22) antibody (PE)
CD22 Reactivity: Human FACS, IFA Host: Mouse Monoclonal RFB4 PE

anti-CD22 Molecule (CD22) (AA 52-178) antibody
CD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat Host: Mouse Monoclonal BLCAM-1796 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 Reactivity: Human, Non-Human Primate FACS, WB, IP Host: Mouse Monoclonal MEM-01 unconjugated

anti-CD22 Molecule (CD22) (AA 708-847) antibody
CD22 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD22 Molecule (CD22) antibody (PE)
CD22 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal MEM-01 PE

anti-CD22 Molecule (CD22) antibody (FITC)
CD22 Reactivity: Human FACS Host: Mouse Monoclonal IS7 FITC

anti-CD22 Molecule (CD22) (AA 20-687) antibody
CD22 Reactivity: Human FACS, WB, ELISA, IF, IP Host: Rabbit Monoclonal DM13 unconjugated

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.
Product Description: CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.

Protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date

24 months
Target

CD22 (CD22 Molecule (CD22))

Alternative Name

CD22

Background

Gene Alias: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-16

UniProt

P20273