Recombinant CD22 antibody (AA 52-178)

Catalog No. ABIN7477403

The Rabbit Monoclonal anti-CD22 antibody has been validated for IHC. It is suitable to detect CD22 in samples from Human.

Quick Overview for Recombinant CD22 antibody (AA 52-178) (ABIN7477403)

Target: CD22 (CD22 Molecule (CD22))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-022R

Product Details anti-CD22 Antibody

Binding Specificity: AA 52-178

Immunogen: Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.

Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P20273