Recombinant Collagen IV antibody

Catalog No. ABIN7477425

This Rabbit Monoclonal antibody specifically detects Collagen IV in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Collagen IV antibody (ABIN7477425)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-704R

Product Details anti-Collagen IV Antibody

Immunogen: Purified human placental extract

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.

Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV

Background: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR,Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02462, P08572, P29400, P53420