Recombinant Collagen IV antibody

Catalog No. ABIN7470593

This Rabbit Monoclonal antibody specifically detects Collagen IV in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Collagen IV antibody (ABIN7470593)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 704R

Product Details anti-Collagen IV Antibody

Purpose: Collagen IV antibody (704R)

Immunogen: Purified human placental extract

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.

Product Description: Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen IV

Background: Gene Alias: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR

UniProt: P02462, P08572, P29400, P53420