Recombinant Prostate Specific Antigen antibody (AA 150-250)

Catalog No. ABIN7477569

This Rabbit Monoclonal antibody specifically detects Prostate Specific Antigen in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Prostate Specific Antigen antibody (AA 150-250) (ABIN7477569)

Target: Prostate Specific Antigen (PSA)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-603R

Product Details anti-Prostate Specific Antigen Antibody

Binding Specificity: AA 150-250

Immunogen: Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.

Negative Control: Colon: PSA immunostaining should be absent in all cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: PSA

Background: Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,PSA antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P07288

Pathways: Complement System