Calpastatin antibody (AA 431-530)

Catalog No. ABIN720161

Product Details anti-Calpastatin Antibody

Target: Calpastatin (CAST)

Binding Specificity: AA 431-530

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpastatin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Calpastatin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Calpastatin antibody (ABIN720161)

Huang, Rose, Hoffmann, Hashimoto, Bertino, Denk, Takano, Iwata, Saido, Hoffmann: "Calpastatin prevents NF-?B-mediated hyperactivation of macrophages and attenuates colitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 7, pp. 3778-88, (2013) (PubMed).

Target Details for Calpastatin

Target: Calpastatin (CAST)

Alternative Name: Calpastatin (CAST Products)

Synonyms: BS-17 antibody, calp3 antibody, CAST antibody, sb:cb966 antibody, zgc:194249 antibody, cast antibody, DKFZp469E0716 antibody, calpastatin antibody, calpastatin S homeolog antibody, Calpastatin antibody, CAST antibody, Cast antibody, cast.S antibody, cast antibody, VDBG_09182 antibody, ical antibody

Background:

Synonyms: BS-17, Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST

Background: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

Gene ID: 831

UniProt: P20810