M. Tuberculosis antibody (FITC)

Catalog No. ABIN7478287

The FITC-conjugated Rabbit Polyclonal anti-M. Tuberculosis antibody (ABIN7478287) specifically detects M. Tuberculosis in Func. The antibody is reactive with Mycobacterium tuberculosis samples.

Quick Overview for M. Tuberculosis antibody (FITC) (ABIN7478287)

Target: M. Tuberculosis

Reactivity: Mycobacterium tuberculosis

Host: Rabbit

Clonality: Polyclonal

Conjugate: FITC

Application: Functional Studies (Func)

Product Details

Specificity: Antibody is reactive with numerous antigens

Cross-Reactivity (Details): This antiserum has not been absorbed & may react with related microorganisms. Antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. Others no tested. Antibody is reactive with the following antigens of M. tuberculosis: Lipoarabinomannan, ESAT 6, CFP10, 38 kDa, Antigen 16 (HspX), Hsp 65 (GroEl) and MoeX. Others not tested.

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: purified PPD

Isotype: IgG

Application Details

Application Notes: TITER : > 1:1,000 by ELISA, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Target Details

Target: M. Tuberculosis