M. Tuberculosis antibody (Peroxidase (POD))

Catalog No. ABIN7478288

This Rabbit Polyclonal antibody specifically detects M. Tuberculosis in IHC, WB and EIA. It exhibits reactivity toward Mycobacterium tuberculosis.

Quick Overview for M. Tuberculosis antibody (Peroxidase (POD)) (ABIN7478288)

Target: M. Tuberculosis

Reactivity: Mycobacterium tuberculosis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Peroxidase (POD)

Application: Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details

Specificity: Antibody is reactive with numerous antigens

Cross-Reactivity (Details): This antiserum has not been absorbed & may react with related microorganisms. Antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. Others no tested. Antibody is reactive with the following antigens of M. tuberculosis: Lipoarabinomannan, ESAT 6, CFP10, 38 kDa, Antigen 16 (HspX), Hsp 65 (GroEl) and MoeX. Others not tested.

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: purified PPD

Isotype: IgG

Application Details

Application Notes: TITER : > 1:1,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details

Target: M. Tuberculosis