Recombinant CD47 antibody

Catalog No. ABIN7487701

This anti-CD47 antibody is a Rabbit Chimeric antibody detecting CD47 in IF and BP. Suitable for Human.

Quick Overview for Recombinant CD47 antibody (ABIN7487701)

Target: CD47

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD47 antibody is un-conjugated

Application: Immunofluorescence (IF), Blocking Peptide (BP)

Clone: MIAP430

Product Details anti-CD47 Antibody

Purpose: Anti-CD47 [mIAP430], Rabbit IgG, kappa

Specificity: This antibody recognises CD47 which is a a multitransmembrane glycoprotein widely expressed on all hematopoietic cells and most other cell types. It is involved in cell adhesion, Ca2+ influx, platelet activation and peripheral mononuclear neutrophils migration across endothelial and epithelial monolayers.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising IAP-deficient mice with purified human placental CD47.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can be used to investigate the role of the CD47 receptor. mIAP430 was first used to visualise the distribution of CD47 on fusing macrophages (Han et al., 2000). Further, it was demonstrated that this antibody can block fusion of macrophages by blocking CD47 and preventing the binding of this receptor with MFR (Han et al., 2000).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: IAP, C5/D5, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6

UniProt: Q08722