Recombinant PAK1 antibody (pSer141, pSer144, pSer154)

Catalog No. ABIN7516572

The Rabbit Monoclonal anti-PAK1 antibody has been validated for WB, IF, IHC (p), IP and IHC (fro). It is suitable to detect PAK1 in samples from Human.

Quick Overview for Recombinant PAK1 antibody (pSer141, pSer144, pSer154) (ABIN7516572)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R04-2A4

Product Details anti-PAK1 Antibody

Binding Specificity: pSer141, pSer144, pSer154

Purpose: Rabbit recombinant monoclonal antibody raised against human PAK1.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1.

Isotype: IgG

Application Details

Application Notes: Immunofluorescence (1:50-1:200),Immunohistochemistry (1:50-1:100),Immunoprecipitation (1:20),Western Blot (1:500-1:1000),The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: P21 protein (Cdc42/Rac)-activated kinase 1

Gene ID: 5058, 5062, 5063

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis