Recombinant ERK1 antibody (pThr185, pThr202)

Catalog No. ABIN7516996

This anti-ERK1 antibody is a Rabbit Monoclonal antibody detecting ERK1 in WB and IP. Suitable for Human.

Quick Overview for Recombinant ERK1 antibody (pThr185, pThr202) (ABIN7516996)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ERK1 Antibody

Binding Specificity: pThr185, pThr202

Purpose: Rabbit recombinant monoclonal antibody raised against human MAPK3/MAPK1.

Specificity: Recognizes endogenous levels of ERK1/2 (pT202/T185) protein.

Cross-Reactivity: Hamster, Human, Rat

Immunogen: Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr185 of human ERK2.

Isotype: IgG

Application Details

Application Notes: Immunoprecipitation(1:10-1:50),Western Blot (1:500-1:1000),The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 50 mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3

Background: Mitogen-activated protein kinase 3

Gene ID: 5595, 5594

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins