ErbB2/Her2 antibody

Catalog No. ABIN7538848

This Mouse Monoclonal antibody specifically detects ErbB2/Her2 in IHC, IHC (p) and HIER. It exhibits reactivity toward Human.

Quick Overview for ErbB2/Her2 antibody (ABIN7538848)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: CB11

Product Details anti-ErbB2/Her2 Antibody

Purpose: Liquid Mouse Monoclonal Antibody c-erbB-2 Oncoprotein

Specificity: Human c-erbB-2 oncoprotein (internal domain). Specific binding has been demonstrated by immunoprecipitation with c-erbB-2 oncoprotein SKBR3 cells.

Characteristics: This product is intended for the qualitative identification by light microscopy of c-erbB-2 Oncoprotein molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Synthetic peptide corresponding to a site on the internal domain of the human c-erbB-2 oncoprotein 8 .

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Not recommended. Suggested dilution: 1:40 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 26 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: c-erbB-2 Oncoprotein

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development