Retinoblastoma 1 antibody (AA 745-928)

Catalog No. ABIN7539642

This Mouse Monoclonal antibody specifically detects Retinoblastoma 1 in WB, ELISA, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for Retinoblastoma 1 antibody (AA 745-928) (ABIN7539642)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 2A11D11

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: AA 745-928

Purpose: RB1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human RB1 (AA: 745-928) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Molecular Weight: 106.2 kDa

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy