H2AFX antibody

Catalog No. ABIN7539773

The Mouse Monoclonal anti-H2AFX antibody (Clone 4A2D5) (ABIN7539773) specifically detects H2AFX in WB, IHC, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for H2AFX antibody (ABIN7539773)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4A2D5

Product Details anti-H2AFX Antibody

Purpose: H2AFX Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human H2AFX expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

FCM: 1/200 - 1/400

ICC: 1/50 - 1/200

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Molecular Weight: 15.1 kDa

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus