5-Hydroxymethylcytosine antibody

Catalog No. ABIN7540500

Supplier Product No.: 200-301-v06

The Mouse Monoclonal anti-5-Hydroxymethylcytosine antibody is suitable to detect 5-Hydroxymethylcytosine in samples from Human. It has been validated for ELISA, DB and FM.

Quick Overview for 5-Hydroxymethylcytosine antibody (ABIN7540500)

Target: 5-Hydroxymethylcytosine (5-hmC)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-5-Hydroxymethylcytosine Antibody

Supplier Product No.: 200-301-v06

Supplier: Rockland

Purpose: 5-hmc Antibody

Cross-Reactivity (Details): Cross-reactivity with 5hmC from other sources has not been determined.

Purification: Anti-5hmC Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Anti-5hmC Antibody was produced in mice by repeated immunization with 5-hydroxymethlcytosine.

Isotype: IgG1 kappa

Application Details

Application Notes:

ELISA_Dilution: 1:500

IF_Microscopy_Dilution: User Optimized

Other: hMeDIP: 2.5 μg per IP. Dot Blot: 2 μg/mL

Comment:

Suggested Applications: hMeDIP: 2.5μg per IP. Dot Blot: 2 μg/ml
Anti-5hmC Antibody is tested for Dot Blotting, ELISA, and Hydroxymethylated DNA Immunoprecipitation Sequencing. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-hydroxymethylcytosine

Target Type: Chemical

Background: 5-hydroxymethylcytosine,5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6 % of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5hmC Antibody is ideal for research in Epigenetics and Gene Expression.