ATM antibody (AA 1974-1988)

Catalog No. ABIN7540580

Supplier Product No.: 600-401-398

This Rabbit Polyclonal antibody specifically detects ATM in WB, IHC and ELISA. It exhibits reactivity toward Human and has been mentioned in 7+ publications.

Quick Overview for ATM antibody (AA 1974-1988) (ABIN7540580)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-ATM Antibody

Binding Specificity: AA 1974-1988

Supplier Product No.: 600-401-398

Supplier: Rockland

Purpose: ATM Protein Kinase S1981 Antibody

Cross-Reactivity (Details): This polyclonal anti-ATM antibody detects native and over-expressed proteins found in various tissues and extracts.

Purification: This affinity-purified antibody is directed against human ATM and is useful in determining its presence in various assays.

Sterility: Sterile filtered

Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide S-L-A-F-E-E-G-S-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM conjugated to KLH using maleimide.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:500- 1:2,000

Other: User Optimized

Comment:

Suggested Applications: FC, IF, Multiplex
Affinity purified rabbit anti-ATM has been tested by ELISA, immunohistochemistry, western blotting and suitable for biological assays against both the native and recombinant forms of the protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ATM antibody (ABIN7540580)

Chang, Liu, Wang, Ma, Zhou, Chen, Sheng, Hu, Du, He, Yang, Zhu: "Hypoxia-Targeted Drug Q6 Induces G2-M Arrest and Apoptosis via Poisoning Topoisomerase II under Hypoxia." in: PloS one, Vol. 10, Issue 12, pp. e0144506, (2019) (PubMed).

Okuno, Nakamura-Ishizu, Otsu, Suda, Kubota: "Pathological neoangiogenesis depends on oxidative stress regulation by ATM." in: Nature medicine, Vol. 18, Issue 8, pp. 1208-16, (2013) (PubMed).

Hasham, Snow, Donghia, Branca, Lessard, Stavnezer, Shopland, Mills: "Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2374-82, (2012) (PubMed).

Muraki, Nabetani, Nishiyama, Ishikawa: "Essential roles of Xenopus TRF2 in telomere end protection and replication." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 16, Issue 6, pp. 728-39, (2011) (PubMed).

Baldeyron, Soria, Roche, Cook, Almouzni: "HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair." in: The Journal of cell biology, Vol. 193, Issue 1, pp. 81-95, (2011) (PubMed).

Chen, Ozanne: "Deep senescent human fibroblasts show diminished DNA damage foci but retain checkpoint capacity to oxidative stress." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6669-73, (2006) (PubMed).

You, Chahwan, Bailis, Hunter, Russell: "ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1." in: Molecular and cellular biology, Vol. 25, Issue 13, pp. 5363-79, (2005) (PubMed).

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: Rabbit anti-ATM antibody, rabbit anti-ATM protein kinase S1891 antibody, rabbit anti-ATM protein kinase antibody, Ataxia telangiectasia mutated, A-T mutated, Serine-protein kinase ATM,ATM, the gene mutated in the hereditary disease ataxia-telangiectasia, codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370- kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint, Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest, and Brca1 and hRad17 in the G2/M checkpoint. See Bakkenist, C. J. & Kastan, M. B. Nature 421, 499-506 (2003) for a complete presentation of this antibody's specificity and utility.

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus