Insulin Receptor antibody (pTyr1361)

Catalog No. ABIN7540640

Supplier Product No.: 600-401-mr0s

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB, IHC, ELISA, DB and FM. It exhibits reactivity toward Human.

Quick Overview for Insulin Receptor antibody (pTyr1361) (ABIN7540640)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-Insulin Receptor Antibody

Binding Specificity: pTyr1361

Supplier Product No.: 600-401-mr0s

Supplier: Rockland

Purpose: Insulin Receptor phospho Y1361 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Insulin Receptor. 

Purification: Rabbit Anti-Insulin Receptor pY1361 Antibody was prepared from monospecific antiserum by delipidation and defibrination, affinity purification, followed by cross adsorption.

Sterility: Sterile filtered

Immunogen: Anti-Insulin Receptor pY1361 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion near the phosphorylation Y1361 of the human Insulin Receptor conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: Dot Blot: 1:1000

Comment:

Anti-Insulin Receptor pY1361 Antibody has been tested in ELISA, Dot Blot, and Western Blot. This antibody may be suitable for Immunofluorescence and Immunohistochemistry. Expect ~156kDa in appropriate tissues or lysates on western blots.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background: Insulin Receptor, EC 2.7.10.1, IR, CD220 Antigen, HHF5, Rabbit anti-Insulin receptor, INSR,Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Anti-Insulin Receptor pY1361 Antibody is useful for researchers interested in Donohue Syndrome, Insulin-Resistant Diabetes Mellitus, RET signaling, AMP-activated Protein Kinase (AMPK) Signaling Pathways, and protein tyrosine kinase activity.

Gene ID: 3643

NCBI Accession: NP_000199

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity